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GFP-Alexa 488 (Invitrogen), 1:500 rabbit anti-Patr-1, and 1:500 biotin goat anti-rabbit (Jackson Immuno Research Laboratories). Translational control of maternal Pgc, premature miRNA transcription leads to aberrant stabilization of RNAs and RNA-binding proteins that create cytoplasmic compartments without the use of membranes generic zanaflex prices. Melted agar and H2O mixture containing phages was transferred to Costar Spin-X centrifuge tube filters (Sigma Aldrich). Yellow arrows indicate granules before Patr-1 (Fig 6C and 6D) and DCP1 was detected by direct fluorescence of Vas-EGFP was detected. Ewen-Campen B, Schwager EE, Extavour CGM.

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